Liam Cooper, Greg Taylor and Jacob Brown have been recalled to the Scotland squad for the Euro 2024 qualifier in Spain.
Hearts striker Lawrence Shankland drops out along with, as expected, Newcastle midfielder Elliot Anderson.
Whitley Bay-born Anderson last week indicated he wanted more time to consider his international future, having left the last Scotland camp after two days following his first call-up to the full squad.
Leeds defender Cooper returns from injury while Celtic left-back Taylor replaces Kieran Tierney, who suffered a hamstring injury while playing for Real Sociedad on Saturday.
Luton striker Brown is yet to score in the Premier League since his move from Stoke, while Shankland has gone seven matches without scoring after hitting five goals in his first six games of the season for Hearts.
Scotland have won their opening five games and are six points ahead of second-placed Spain ahead of their trip to Seville on October 12.
Steve Clarke’s side then face France in a friendly in Lille five days later.
Scotland Squad in full
Goalkeepers
Zander Clark Heart of Midlothian
Angus Gunn Norwich City
Liam Kelly Motherwell
Defenders
Liam Cooper Leeds United
Jack Hendry Al-Ettifaq
Aaron Hickey Brentford
Scott McKenna Nottingham Forest
Nathan Patterson* Everton
Ryan Porteous Watford
Andy Robertson Liverpool
John Souttar Rangers
Greg Taylor Celtic
Midfielders
Stuart Armstrong Southampton
Lewis Ferguson Bologna
Billy Gilmour* Brighton
Ryan Jack Rangers
John McGinn Aston Villa
Callum McGregor Celtic
Kenny McLean Norwich City
Scott McTominay Manchester United
Forwards
Ché Adams Southampton
Jacob Brown Luton Town
Ryan Christie Bournemouth
Lyndon Dykes Queens Park Rangers
Kevin Nisbet Millwall
*Denotes Scottish FA JD Performance School graduate
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