Boris Johnson was booed by protesters in Edinburgh as he arrived at Bute House for talks with Nicola Sturgeon.
Mr Johnson was jeered as he shook hands with the First Minister outside her official residence in Edinburgh.
Independence campaigners and pro-EU demonstrations gathered across the street ahead of Mr Johnson's arrival - though at least one pro-Boris supporter was among the crowd.
One lone figure pushing against the EU/Yes flags outside Bute House. pic.twitter.com/VcTIMSpbAs
— Alistair Grant (@alistairkgrant) July 29, 2019
The frosty reception came after the new prime minister declared he saw "no reason" for Scots to hold a second independence referendum.
Earlier at Faslane Naval Base, he also appeared unsure about how many referendums on Scottish independence there have been.
Mr Johnson faced fraught discussions with Ms Sturgeon and Scottish Tory leader Ms Davidson, who both vowed to oppose a no-deal Brexit.
Ms Sturgeon today accused him of planning a "catastrophic no-deal Brexit" and demanded that Scotland be allowed to 'chart its own course' with another independence referendum.
Mr Johnson insisted that the 2014 vote had been "once in a generation".
At his meeting with Nicola Sturgeon, he also said that the UK would leave the EU on 31 October "come what may".
A Number 10 spokesman said: "The Prime Minister met the First Minister for Scotland Nicola Sturgeon in Edinburgh today.
"The Prime Minister said he was a passionate believer in the power of the Union and he would work tirelessly to strengthen the United Kingdom and improve the lives of people right across Scotland.
"On Brexit, the Prime Minister said that while the Government's preference is to negotiate a new deal which abolishes the anti-democratic backstop, the UK will be leaving the EU on October 31st come what may."
Earlier in the day Mr Johnson met Ruth Davidson for a one-on-one at Holyrood.
Singing her praises before themeeting, the Prime Minister described her as a "fantastic leader".
Scottish Conservative leader @RuthDavidsonMSP welcomed Prime Minister @BorisJohnson to the Scottish Parliament earlier today. pic.twitter.com/zlRgje8kpC
— ScotConservatives (@ScotTories) July 29, 2019
Speaking at Faslane, he said: "I am lost in admiration at what she has achieved, I am a massive fan of the way she has taken the argument to those who would destroy our union."
But Ms Davidson described their encounter as "incredibly constructive".
She said: “We covered a number of areas, talking about Brexit, the need to make sure we can get a deal across the line, and I support the Prime Minister wholeheartedly in getting that deal.
“We talked about how we can continue to have the UK government deliver in Scotland, for Scots, and how we can build on that delivery, and also how we can continue to take on Nicola Sturgeon and oppose her obsession with another independence referendum.”
Boris in Scotland
- Johnson appears unsure of number of Scottish independence votes
- PM tries to heal Brexit rift with "fantastic" Ruth Davidson
- Johnson sees ‘no reason’ for Scotland holding second independence vote
- Sturgeon tells Johnson: Brexit offers 'no democracy' for Scotland
- Johnson pledges to strengthen Union as he prepares for clash with Ruth Davidson
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