A humiliated Theresa May has suffered a second defeat after MPs rejected her bid to secure a Brexit deal for the second time.
The revised Brexit deal was defeated by 391 to 242 - a majority of 149.
Although the 149 margin was reduced from the record 230-vote defeat of the first "meaningful vote" in January, Mrs May was left far adrift from a majority with just 17 days to go to the scheduled date of Brexit on March 29.
European Commission president Mr Juncker had already warned that if MPs turned down the package agreed in Strasbourg on Monday, there would be "no third chance" to renegotiate.
In line with a promise set out by Mrs May last month, MPs are now due to vote on Wednesday on whether they are willing for the UK to leave the EU without a deal on March 29.
If they reject no-deal as most Westminster observers expect, a third vote will follow - probably on Thursday - on authorising Mrs May to request an extension of the two-year Article 50 negotiation process.
Read more: Brexit vote: What happens now?
An extension requires the unanimous agreement of all 27 remaining member states, and Mr Juncker has warned that it cannot stretch beyond May 23 unless the UK takes part in the European Parliament elections starting on that date.
Cabinet gave its approval to Mrs May's package at an early-morning meeting in 10 Downing Street which ended with the PM telling colleagues: "Today is the day. Let's get this done."
But the momentum moved sharply against the Prime Minister shortly afterwards, as Attorney General Geoffrey Cox released formal legal advice that the changes secured by Mrs May "reduce the risk" that the backstop will be permanent, but do not remove it altogether.
The Star Chamber of lawyers convened by the Brexit-backing European Research Group declared that three new documents agreed in Strasbourg failed to deliver the legally-binding changes demanded by the Commons.
And the Democratic Unionist Party - which props up Mrs May's minority administration in the Commons - said its 10 MPs would vote against the latest deal as "sufficient progress has not been achieved at this time".
The Prime Minister, battling a croaky voice and with husband Philip watching from the Commons gallery, warned MPs that "Brexit could be lost" if they gave her deal the thumbs-down again.
Read more: Iain Macwhirter: End of the road for May and perhaps the end of Brexit
But she met a wall of hostility from opposition parties, while only a handful of the 118 Tories who rebelled in January said they would switch sides to back her.
Shadow Brexit secretary Sir Keir Starmer said that "no significant changes" had been secured in two months of negotiations and the Government's strategy was "in tatters".
Brexit figurehead and former foreign secretary Boris Johnson told the Commons that Mrs May and Mr Cox had "sowed an apron of fig leaves that does nothing to conceal the embarrassment and indignity of the UK."
Labour leader Jeremy Corbyn told the Commons: "After three months of running down the clock the Prime Minister has, despite very extensive delays, achieved not a single change to the Withdrawal Agreement.
"Not one single word has changed. In terms of the substance, literally, nothing has changed."
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