The First Minister has said she is "extremely disappointed" over industrial action at ScotRail.
The RMT union has announced a fresh campaign of industrial action over plans to allow drivers, rather than a conductor, to operate doors on trains.
The union fears the introduction of driver-operated doors will "water down and wipe out the safety critical role of the guard on these ScotRail services".
Nicola Sturgeon stressed that ScotRail's franchise mandates at least two members of staff on a train at all times, and insisted driver-operated doors have been in operation for at least 30 years.
READ MORE: ScotRail workers to launch fresh strikes in driver-only train row
Speaking at First Minister's Questions, she said: "I'm extremely disappointed that we're seeing industrial action on our railways.
"It is, after all, the travelling public that loses out in situations like this so I would once again urge both parties to reopen meaningful discussions and work towards an agreement that stops further strikes from going ahead.
"Safety is paramount to everything that is done in our rail network."
She added: "A multi-agency response team has and will continue to operate on each of the strike dates, if they go ahead, and all modes of transport have continued to perform well."
READ MORE: ScotRail workers to launch fresh strikes in driver-only train row
Labour MSP Neil Bibby said: "Conductor operation guarantees passengers that a rail worker, in addition to the driver, will always be on a train to assist passengers, including in the event of an emergency."
He asked whether passengers will be at risk if there is an accident and whether disabled people would be disadvantaged if "there is no longer a second rail worker to assist".
Ms Sturgeon said Labour has displayed "a complete misunderstanding of the issue".
She said: "The franchise mandates ScotRail to have that second member of staff, unless there are exceptional circumstances, on board each and every single train and that is audited regularly.
READ MORE: ScotRail workers to launch fresh strikes in driver-only train row
"This not an issue about whether there is no longer going to be a second member of staff on the train, this is an issue about whether it is drivers that open the doors or not."
She said drivers have been able to operate doors for at least 30 years and urged Labour to stop spreading "the wrong information".
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