Charlotte Avenall, who had severe learning difficulties, was found dead in her bedroom in Mansfield, Nottinghamshire last month.
Simon and Susan Moody were interviewed under caution immediately after finding Charlotte Avenall's body at their home in Mansfield, Nottinghamshire, last month.
The young girl, who had severe learning disabilities, was discovered hanged in her bedroom on September 12.
Mr Moody, 32, and his 24-year-old wife Susan were arrested today in connection with her death.
A spokeswoman for Nottinghamshire Police said: "A 32-year-old man and a 24-year-old woman were arrested today in connection with the investigation into the circumstances surrounding the death of eight-year-old Charlotte Avenall, from Mansfield.
"They are currently being questioned by police."
Police said at the time of the tragedy that she had been discovered by her parents and they had been interviewed under caution shortly afterwards.
Nottinghamshire County Council has already launched a full investigation into the circumstances surrounding Charlotte’s death.
The local authority’s social services department would be reviewing the care and support it offered Charlotte and her family.
She was known to social services from birth and attended Birklands Primary School in nearby Warsop.
Before the development, Chief Inspector Paul Winter, the officer involved in the investigation into the death, had described the death as “tragic and untimely.”
A post mortem confirmed the cause of death as hanging.
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